Pauka Hesperidin

ʻO Hesperidin kahi glycoside dihydroflavonoid me ke ʻano molekala C29H33O15 a me ke kaumaha molekala o 610.56. ʻIke ʻia ka hesperidin maʻemaʻe e like me nā kristal keʻokeʻo e like me ke kui, ʻoiai ʻo ka mea i unuhi ʻia he pauka crystalline ʻeleʻele. Ua hoʻokaʻawale mua ʻia mai ka ʻili citrus i ka makahiki 1828, ʻo ia ka mea hana nui o ka huaora P (vitamin permeability), ka mea i loaʻa ai ka inoa "vitamin P kūlohelohe". Hoʻolaha nui ʻia ʻo Hesperidin ma ke ʻano: 30%–50% i loaʻa i loko o ka ʻili, pericarp a me ka ʻili o nā hua citrus maloʻo, a ma kahi o 1%–5% i loaʻa i loko o ka ʻili. Hoʻohana ka hana ʻoihana hou i nā ʻōpala hana hua citrus, nā ʻili a me nā pericarps ma ke ʻano he mea maka. Ma ka unuhi alkaline a me ka hoʻoheheʻe ʻakika, ka unuhi ʻana i kōkua ʻia e ka ultrasonic a i ʻole ka hoʻololi ion, ua hoʻopaʻa ʻia ka hesperidin ma mua o 90% a laila hoʻomaloʻo ʻia i loko o ka pauka hesperidin wai.

Wai: Ma kahi o 50 lita o ka wai maʻemaʻe ma 25°C e pono ai e hoʻoheheʻe loa i 1 gram o ka pauka hesperidin; piʻi iki ka solubility i ka wai wela ma luna o 70°C.

Nā mea hoʻoheheʻe organik: Hoʻoheheʻe maikaʻi ʻia i loko o ka pyridine, dimethylformamide a me ka hopena sodium hydroxide; hoʻoheheʻe iki ʻia i loko o ka waikawa acetic glacial a me ka methanol; hoʻoheheʻe ʻole ʻia i loko o ka acetone, benzene, chloroform a me ka ether.

ʻike pH: I loko o nā hopena alkaline (pH > 9), wehe ia me ke apo e hana iā lākou, kahi e hoʻonui koke ai i kona solubility. Ke hoʻi ka pH i nā pae acidic, pani hou ia a pehu. No laila, ʻo ka "alkaline dissolution and acid precipitation" kahi ʻano hana maʻamau no ka hoʻomaʻemaʻe ʻana i ka pauka hesperidine ma ka hale hana.

Ke koʻikoʻi o ke kaʻina hana: Ma muli o kona hiki ʻole ke hoʻoheheʻe ʻia i ka wai, maʻalahi ka pauka hesperidine maʻamau i loko o nā ʻōnaehana wai nui e like me nā mea inu a me nā wai waha. ʻO ka hoʻohui ʻana o ka hydroxypropyl-β-cyclodextrin, ka dispersion paʻa, a i ʻole nā ​​​​​​ʻano hana microemulsification e hoʻohana pinepine ʻia i ka ʻoihana e hoʻomaikaʻi i kona kūpaʻa dispersion wai a hōʻoia i ka maʻemaʻe a me ka maopopo o ka huahana hope loa.

No nā ʻōnaehana inoa kahiko a me nā ʻōnaehana inoa hou nā "Glycosides" a me nā "glycosides":
• Nā inoa kahiko: Ua unuhi ʻia ʻo "Glycosides" i loko o ka hesperidin mai ka ʻōlelo Kepanī mai i nā makahiki 1950;
• Inoa hou: Ma hope o nā makahiki 1990, ua hoʻololi ke Kōmike ʻŌlelo ʻEpekema o koʻu ʻāina i ka huaʻōlelo "glycosides" i kahi huaʻōlelo like no "glycosides", a ʻo ka hopena o ka inoa "hesperidin".

ʻO ka hopena, ma nā puke pai hoʻonaʻauao, nā pharmacopoeias a me nā kūlana honua, ua kākau ʻia ʻo ia i kēia manawa ʻo "hesperidin" ma ke ʻano like. Eia nō naʻe, ke hoʻohana mau nei kekahi mau palapala kahiko a me kekahi mau ʻenekinia kahiko i ka huaʻōlelo "hesperidin". Loaʻa i nā mea ʻelua ke ʻano kemika like a me nā helu CAS (520-26-3), a hiki ke manaʻo ʻia he mea like lākou. He mea nui e hoʻomaopopo ʻo nā huaʻōlelo "Hesperidin = Hesperidin-7-O-rutinoside" a me "Hesperidin = Hesperidin-7-O-neohesperidin" āu e ʻike ai ma ka pūnaewele he mau wehewehe hewa ia o "Hesperidin a me Neohesperidin" a ʻaʻole pono e huikau.

Inā ʻoe e ʻimi nei i ka pauka hesperidine papa meaʻai a i ʻole USP, e ʻoluʻolu e kelepona iā SOST Biotech, kahi mea hana ʻoihana mea kanu. Aia ke keʻena hale hana ma ka ʻāpana ʻenehana kiʻekiʻe o Xi'an. Loaʻa iā ia kahi keʻena hoʻokolohua GMP a me nā keʻena hoʻokolohua HPLC a me GC-MS piha. ʻEkolu mau kikoʻī kona maʻamau: 90%, 95%